Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease

Giorgio Vivacqua, Anna Latorre, Antonio Suppa, Michela Nardi, Sara Pietracupa, Romina Mancinelli, Giovanni Fabbrini, Carlo Colosimo, Eugenio Gaudio, Alfredo Berardelli

Research output: Contribution to journalArticle

  • 9 Citations

Abstract

In Parkinson's disease (PD), alpha-synuclein (a-syn) can be detected in biological fluids including saliva. Although previous studies found reduced a-syn total (a-syntotal) concentration in saliva of PD patients, no studies have previously examined salivary a-syn oligomers (a-synolig) concentrations or assessed the correlation between salivary a-syntotal, a-synolig and clinical features in a large cohort of PD patients. Is well known that a-synolig exerts a crucial neurotoxic effect in PD. We collected salivary samples from 60 PD patients and 40 age- and sex-comparable healthy subjects. PD was diagnosed according to the United Kingdom Brain Bank Criteria. Samples of saliva were analyzed by specific anti-a-syn and anti-oligomeric a-syn ELISA kits. A complete clinical evaluation of each patient was performed using MDS-Unified Parkinson's Disease Rating Scale, Beck Depression Inventory, Montreal Cognitive Assessment and Frontal Assessment Battery. Salivary a-syntotal was lower, whereas a-synolig was higher in PD patients than healthy subjects. The a-synolig/asyntotal ratio was also higher in patients than in healthy subjects. Salivary a-syntotal concentration negatively correlated with that of a-synolig and correlated with several patients' clinical features. In PD, decreased salivary concentration of a-syntotal may reflect the reduction of a-syn monomers (a-synmon), as well as the formation of insoluble intracellular inclusions and soluble oligomers. The combined detection of a-syntotal and a-synolig in the saliva might help the early diagnosis of PD.

Original languageEnglish
Article numbere0151156
JournalPLoS One
Volume11
Issue number3
DOIs
StatePublished - 1 Mar 2016

Fingerprint

alpha-Synuclein
Parkinson Disease
Parkinson disease
Supravalvular Aortic Stenosis
Saliva
saliva
Healthy Volunteers
sampling
Filaricides
Early Diagnosis
Enzyme-Linked Immunosorbent Assay
Equipment and Supplies
Brain
rating scales
early diagnosis
enzyme-linked immunosorbent assay
brain
liquids
gender
Erysipeloid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Vivacqua, G., Latorre, A., Suppa, A., Nardi, M., Pietracupa, S., Mancinelli, R., ... Berardelli, A. (2016). Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease. PLoS One, 11(3), [e0151156]. DOI: 10.1371/journal.pone.0151156

Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease. / Vivacqua, Giorgio; Latorre, Anna; Suppa, Antonio; Nardi, Michela; Pietracupa, Sara; Mancinelli, Romina; Fabbrini, Giovanni; Colosimo, Carlo; Gaudio, Eugenio; Berardelli, Alfredo.

In: PLoS One, Vol. 11, No. 3, e0151156, 01.03.2016.

Research output: Contribution to journalArticle

Vivacqua, G, Latorre, A, Suppa, A, Nardi, M, Pietracupa, S, Mancinelli, R, Fabbrini, G, Colosimo, C, Gaudio, E & Berardelli, A 2016, 'Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease' PLoS One, vol 11, no. 3, e0151156. DOI: 10.1371/journal.pone.0151156
Vivacqua G, Latorre A, Suppa A, Nardi M, Pietracupa S, Mancinelli R et al. Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease. PLoS One. 2016 Mar 1;11(3). e0151156. Available from, DOI: 10.1371/journal.pone.0151156

Vivacqua, Giorgio; Latorre, Anna; Suppa, Antonio; Nardi, Michela; Pietracupa, Sara; Mancinelli, Romina; Fabbrini, Giovanni; Colosimo, Carlo; Gaudio, Eugenio; Berardelli, Alfredo / Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease.

In: PLoS One, Vol. 11, No. 3, e0151156, 01.03.2016.

Research output: Contribution to journalArticle

@article{a45424a18b8f4eee82ad86f0f6b32f34,
title = "Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease",
abstract = "In Parkinson's disease (PD), alpha-synuclein (a-syn) can be detected in biological fluids including saliva. Although previous studies found reduced a-syn total (a-syntotal) concentration in saliva of PD patients, no studies have previously examined salivary a-syn oligomers (a-synolig) concentrations or assessed the correlation between salivary a-syntotal, a-synolig and clinical features in a large cohort of PD patients. Is well known that a-synolig exerts a crucial neurotoxic effect in PD. We collected salivary samples from 60 PD patients and 40 age- and sex-comparable healthy subjects. PD was diagnosed according to the United Kingdom Brain Bank Criteria. Samples of saliva were analyzed by specific anti-a-syn and anti-oligomeric a-syn ELISA kits. A complete clinical evaluation of each patient was performed using MDS-Unified Parkinson's Disease Rating Scale, Beck Depression Inventory, Montreal Cognitive Assessment and Frontal Assessment Battery. Salivary a-syntotal was lower, whereas a-synolig was higher in PD patients than healthy subjects. The a-synolig/asyntotal ratio was also higher in patients than in healthy subjects. Salivary a-syntotal concentration negatively correlated with that of a-synolig and correlated with several patients' clinical features. In PD, decreased salivary concentration of a-syntotal may reflect the reduction of a-syn monomers (a-synmon), as well as the formation of insoluble intracellular inclusions and soluble oligomers. The combined detection of a-syntotal and a-synolig in the saliva might help the early diagnosis of PD.",
author = "Giorgio Vivacqua and Anna Latorre and Antonio Suppa and Michela Nardi and Sara Pietracupa and Romina Mancinelli and Giovanni Fabbrini and Carlo Colosimo and Eugenio Gaudio and Alfredo Berardelli",
year = "2016",
month = "3",
doi = "10.1371/journal.pone.0151156",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

TY - JOUR

T1 - Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease

AU - Vivacqua,Giorgio

AU - Latorre,Anna

AU - Suppa,Antonio

AU - Nardi,Michela

AU - Pietracupa,Sara

AU - Mancinelli,Romina

AU - Fabbrini,Giovanni

AU - Colosimo,Carlo

AU - Gaudio,Eugenio

AU - Berardelli,Alfredo

PY - 2016/3/1

Y1 - 2016/3/1

N2 - In Parkinson's disease (PD), alpha-synuclein (a-syn) can be detected in biological fluids including saliva. Although previous studies found reduced a-syn total (a-syntotal) concentration in saliva of PD patients, no studies have previously examined salivary a-syn oligomers (a-synolig) concentrations or assessed the correlation between salivary a-syntotal, a-synolig and clinical features in a large cohort of PD patients. Is well known that a-synolig exerts a crucial neurotoxic effect in PD. We collected salivary samples from 60 PD patients and 40 age- and sex-comparable healthy subjects. PD was diagnosed according to the United Kingdom Brain Bank Criteria. Samples of saliva were analyzed by specific anti-a-syn and anti-oligomeric a-syn ELISA kits. A complete clinical evaluation of each patient was performed using MDS-Unified Parkinson's Disease Rating Scale, Beck Depression Inventory, Montreal Cognitive Assessment and Frontal Assessment Battery. Salivary a-syntotal was lower, whereas a-synolig was higher in PD patients than healthy subjects. The a-synolig/asyntotal ratio was also higher in patients than in healthy subjects. Salivary a-syntotal concentration negatively correlated with that of a-synolig and correlated with several patients' clinical features. In PD, decreased salivary concentration of a-syntotal may reflect the reduction of a-syn monomers (a-synmon), as well as the formation of insoluble intracellular inclusions and soluble oligomers. The combined detection of a-syntotal and a-synolig in the saliva might help the early diagnosis of PD.

AB - In Parkinson's disease (PD), alpha-synuclein (a-syn) can be detected in biological fluids including saliva. Although previous studies found reduced a-syn total (a-syntotal) concentration in saliva of PD patients, no studies have previously examined salivary a-syn oligomers (a-synolig) concentrations or assessed the correlation between salivary a-syntotal, a-synolig and clinical features in a large cohort of PD patients. Is well known that a-synolig exerts a crucial neurotoxic effect in PD. We collected salivary samples from 60 PD patients and 40 age- and sex-comparable healthy subjects. PD was diagnosed according to the United Kingdom Brain Bank Criteria. Samples of saliva were analyzed by specific anti-a-syn and anti-oligomeric a-syn ELISA kits. A complete clinical evaluation of each patient was performed using MDS-Unified Parkinson's Disease Rating Scale, Beck Depression Inventory, Montreal Cognitive Assessment and Frontal Assessment Battery. Salivary a-syntotal was lower, whereas a-synolig was higher in PD patients than healthy subjects. The a-synolig/asyntotal ratio was also higher in patients than in healthy subjects. Salivary a-syntotal concentration negatively correlated with that of a-synolig and correlated with several patients' clinical features. In PD, decreased salivary concentration of a-syntotal may reflect the reduction of a-syn monomers (a-synmon), as well as the formation of insoluble intracellular inclusions and soluble oligomers. The combined detection of a-syntotal and a-synolig in the saliva might help the early diagnosis of PD.

UR - http://www.scopus.com/inward/record.url?scp=84962069192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962069192&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0151156

DO - 10.1371/journal.pone.0151156

M3 - Article

VL - 11

JO - PLoS One

T2 - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 3

M1 - e0151156

ER -