Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures

Alessio Valletta, Giulia De Angelis, Camilla Badiali, Elisa Brasili, Alfredo Miccheli, Maria Enrica Di Cocco, Gabriella Pasqua

Research output: Contribution to journalArticle

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Abstract

Key message: Acetic acid acts as a signal molecule, strongly enhancing xanthone biosynthesis inHypericum perforatumroot cultures. This activity is specific, as demonstrated by the comparison with other short-chain monocarboxylic acids. Abstract: We have recently demonstrated that Hypericum perforatum root cultures constitutively produce xanthones at higher levels than the root of the plant and that they respond to chitosan (CHIT) elicitation with a noteworthy increase in xanthone production. In the present study, CHIT was administered to H. perforatum root cultures using three different elicitation protocols, and the increase in xanthone production was evaluated. The best results (550 % xanthone increase) were obtained by subjecting the roots to a single elicitation with 200 mg l−1 CHIT and maintaining the elicitor in the culture medium for 7 days. To discriminate the effect of CHIT from that of the solvent, control experiments were performed by administering AcOH alone at the same concentration used for CHIT solubilization. Unexpectedly, AcOH caused an increase in xanthone production comparable to that observed in response to CHIT. Feeding experiments with 13C-labeled AcOH demonstrated that this compound was not incorporated into the xanthone skeleton. Other short-chain monocarboxylic acids (i.e., propionic and butyric acid) have little or no effect on the production of xanthones. These results indicate that AcOH acts as a specific signal molecule, able to greatly enhance xanthone biosynthesis in H. perforatum root cultures.

Original languageEnglish
Pages (from-to)1009-1020
Number of pages12
JournalPlant Cell Reports
Volume35
Issue number5
DOIs
StatePublished - 1 May 2016

Fingerprint

xanthone
Hypericum
Chitosan
Acetic Acid
chitosan
Hypericum perforatum
biosynthesis
Xanthones
monocarboxylic acids
xanthones
acetic acid
elicitors
Plant Roots
Butyric Acid
Propionates
Culture Media
Acids
butyric acid
propionic acid
solubilization

Keywords

  • Acetic acid
  • Chitosan
  • Elicitor
  • Hypericum perforatum
  • Root cultures
  • Xanthones

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Valletta, A., De Angelis, G., Badiali, C., Brasili, E., Miccheli, A., Di Cocco, M. E., & Pasqua, G. (2016). Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures. Plant Cell Reports, 35(5), 1009-1020. DOI: 10.1007/s00299-016-1934-x

Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures. / Valletta, Alessio; De Angelis, Giulia; Badiali, Camilla; Brasili, Elisa; Miccheli, Alfredo; Di Cocco, Maria Enrica; Pasqua, Gabriella.

In: Plant Cell Reports, Vol. 35, No. 5, 01.05.2016, p. 1009-1020.

Research output: Contribution to journalArticle

Valletta, A, De Angelis, G, Badiali, C, Brasili, E, Miccheli, A, Di Cocco, ME & Pasqua, G 2016, 'Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures' Plant Cell Reports, vol 35, no. 5, pp. 1009-1020. DOI: 10.1007/s00299-016-1934-x
Valletta A, De Angelis G, Badiali C, Brasili E, Miccheli A, Di Cocco ME et al. Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures. Plant Cell Reports. 2016 May 1;35(5):1009-1020. Available from, DOI: 10.1007/s00299-016-1934-x

Valletta, Alessio; De Angelis, Giulia; Badiali, Camilla; Brasili, Elisa; Miccheli, Alfredo; Di Cocco, Maria Enrica; Pasqua, Gabriella / Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures.

In: Plant Cell Reports, Vol. 35, No. 5, 01.05.2016, p. 1009-1020.

Research output: Contribution to journalArticle

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