Acetonic Extract from the Feijoa sellowiana Berg. Fruit Exerts Antioxidant Properties and Modulates Disaccharidases Activities in Human Intestinal Epithelial Cells

Fabio Turco, Ilaria Palumbo, Paolo Andreozzi, Giovanni Sarnelli, Francesca De Ruberto, Giuseppe Esposito, Adriana Basile, Rosario Cuomo

Research output: Contribution to journalArticle

Abstract

Feijoa sellowiana fruit has been shown to possess various biological activities, such as anti-bacterial and anti-cancer properties, in a variety of cellular models, but its activity on human intestinal epithelial cells has never been tested. The purpose of this study was to investigate the effects of the acetonic extract of F. sellowiana fruits on the viability, membrane peroxidation, disaccharidases activities and proliferation of in vitro models of human intestinal epithelial cells. To obtain this goal, Caco-2 and HT-29 cells were exposed to the acetonic extract for 24h. Cell proliferation, viability, lactase and sucrase-isomaltase activity and H2O2-induced membrane lipid peroxidation were tested. We found that, compared to control conditions, the acetonic extract significantly increased lactase and sucrase-isomaltase activity in Caco-2, but not HT-29, cells, decreased proliferation, had no effects on viability and restored lipid peroxidation in both cell models. This study suggests that the acetonic extract improves lactase and sucrase-isomaltase activity, inhibits cell proliferation, have no cytotoxic effects and prevent lipid peroxidation of intestinal epithelial cells. These effects may be exploited in case of disaccharidases deficit and also as an adjuvant treatment of diseases related to oxidative stress.

Original languageEnglish
JournalPhytotherapy Research
DOIs
StateAccepted/In press - 2016

Fingerprint

Feijoa
Oligo-1,6-Glucosidase
Disaccharidases
Sucrase
Lactase
Lipid Peroxidation
Fruit
Antioxidants
Epithelial Cells
Cell Proliferation
HT29 Cells
Membrane Lipids
Cell Survival
Oxidative Stress
Membranes
Neoplasms
In Vitro Techniques

Keywords

  • Disaccharidases deficiency
  • Functional foods
  • Lactose malabsorption
  • Oxidative stress
  • Sucrase-isomaltase deficiency

ASJC Scopus subject areas

  • Pharmacology

Cite this

Acetonic Extract from the Feijoa sellowiana Berg. Fruit Exerts Antioxidant Properties and Modulates Disaccharidases Activities in Human Intestinal Epithelial Cells. / Turco, Fabio; Palumbo, Ilaria; Andreozzi, Paolo; Sarnelli, Giovanni; De Ruberto, Francesca; Esposito, Giuseppe; Basile, Adriana; Cuomo, Rosario.

In: Phytotherapy Research, 2016.

Research output: Contribution to journalArticle

Turco, Fabio; Palumbo, Ilaria; Andreozzi, Paolo; Sarnelli, Giovanni; De Ruberto, Francesca; Esposito, Giuseppe; Basile, Adriana; Cuomo, Rosario / Acetonic Extract from the Feijoa sellowiana Berg. Fruit Exerts Antioxidant Properties and Modulates Disaccharidases Activities in Human Intestinal Epithelial Cells.

In: Phytotherapy Research, 2016.

Research output: Contribution to journalArticle

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